Orchestrating Spatial Transcriptomics Analysis with Bioconductor
Helena Lucia CrowelliD, Yixing DongiD, Ilaria BillatoiD, Peiying CaiiD, Martin EmonsiD, Samuel GunziD, Boyi GuoiD, Mengbo LiiD, Alexandru MahmoudiD, Artür ManukyaniD, Hervé PagèsiD, Pratibha PanwariD, Shreya RaoiD, Callum J. SargeantiD, Lori A. ShepherdiD, Marcel RamosiD, Jieran SuniD, Michael S. TottyiD, Vincent J. CareyiD, Yunshun CheniD, Leonardo Collado‐TorresiD, Shila GhazanfariD, Kasper D. HanseniD, Keri MartinowichiD, Kristen R. MaynardiD, , Lukas M. WeberiD
Abstract
Spatial transcriptomics technologies provide spatially-resolved measurements of gene expression through assays that can either target selected genes or capture transcriptome-wide expression profiles. The complexity and variability of these technologies and their associated data necessitate multi-step workflows integrating diverse computational methods and software packages. We provide a freely accessible, open-source, continuously updated and tested online book containing reproducible code examples, datasets, and discussion about data analysis workflows for spatial omics data using Bioconductor in R, including interoperability with Python.
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