bioRxivCell Biology
Enhanced Single RNA Imaging Reveals Dynamic Gene Expression in Live Animals
Abstract
Imaging endogenous mRNAs in live animals is technically challenging. Here we describe an MS2 based signal Amplification with Suntag System that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1,300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- An adapted MS2-MCP system to visualize endogenous cytoplasmic mRNA with live imaging in Caenorhabditis elegans
Cristina Tocchini, Susan E. Mango·bioRxiv·Jun 13, 2023
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain
Hyungsik Lim, Grace P Cooper·bioRxiv·Feb 5, 2026
- Single-molecule live-cell RNA imaging with CRISPR-Csm
Chenglong Xia, David Colognori, … Jennifer A. Doudna·Nature BiotechnologyPeer-reviewed·Feb 18, 2025
- SunRISE: long-term imaging of individual mRNA molecules in living cells
Y. Guo, R. E. C. Lee·Cell Reports MethodsPeer-reviewed·Jun 1, 2022
- Background free tracking of single RNA in living cells using catalytically inactive CasE
Feng Gao, Yue Sun, … Chunyu Han·bioRxiv·May 13, 2019
- Nuclear-BRITE: an enhanced MS2 system for live-cell imaging of nuclear RNAs
Adam D. Cawte, Bara Cihlova, … Neil Brockdorff·bioRxiv·Oct 9, 2026