bioRxivMolecular BiologyPreprint
Background free tracking of single RNA in living cells using catalytically inactive CasE
Feng GaoiD, Yue Sun, Feng JiangiD, Xiaoyue Bai, Chunyu HaniD
Abstract
RNAs have important and diverse functions. Visualizing an isolated RNA in living cells provide us essential information of its roles. By now, there are two kinds of live RNA imaging systems invented, one is the MS2 system and the other is the Cas13a system. In this study, we show that when fused with split-Fp, CasE can be engineered into a live RNA tracking tool.
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