Susan E. Mango

Safeguarding epithelial junctions by a novel quality control pathway

The paper identifies a novel quality control pathway (J-QC) that maintains epithelial junction integrity by regulating DLG-1 protein levels and responding to disruptions in the junctional complex.

Whole-embryo Spatial Transcriptomics at Subcellular Resolution from Gastrulation to Organogenesis

The paper introduces a whole-embryo spatial transcriptomics method and generates a comprehensive gene expression and chromatin accessibility atlas in zebrafish embryos.

The One Click Wonder: a retrained automated segmentation pipeline that enables quantitative and modular analysis of C. elegans embryos

The paper introduces OCW, an automated segmentation pipeline for C. elegans embryos, and BAAM for single-cell quantitation, revealing distinct cell populations with varying transcriptional burst frequencies.

Rapid canalization of chromosome conformation-transcription fingerprints during embryogenesis revealed by fully-automated cell identity decoding with CeSCALE

The paper develops CeSCALE to analyze lineage-resolved conformation-transcription relationships in C. elegans embryos, revealing robust inheritance of local chromatin features during development.

Nuclear lamina-associated domain biogenesis is regulated by nuclear pore density during embryogenesis and mediates UV protection

The paper shows that nuclear pore density regulates LAD formation during C. elegans embryogenesis, with heterochromatic LADs providing UV protection.

Single-molecule chromosome tracing reveals a diversity of megabase heterochromatin domains

The paper identifies three types of megabase-scale heterochromatin domains in C. elegans using single-molecule tracing and shows their developmental dynamics and structural differences.

Differential functions of upstream and intronic HOT regions shape dlg-1 transcription and C. elegans development

The paper identifies two HOT regions in the C. elegans dlg-1 gene, showing the upstream region is essential for transcription and development, while the intronic region modestly enhances transcription in epidermal cells.

The mechanosensory DEG/ENaC channel DEGT-1 is a proprioceptor of C. elegans foregut movement

This study identifies DEGT-1 as a mechanosensory ion channel in C. elegans that modulates pharyngeal pumping by detecting proprioceptive feedback from organ movement.

HPL-2/HP1 and MET-2/SETDB1 bind distinct co-factors that promote heterochromatic foci, gene repression and organogenesis independently of H3K9 methylation

The paper shows HPL-2 and MET-2 function in parallel H3K9me-independent pathways for gene repression and organogenesis, using distinct co-factors.

An adapted MS2-MCP system to visualize endogenous cytoplasmic mRNA with live imaging in Caenorhabditis elegans

The paper adapts an MS2-MCP system to visualize endogenous cytoplasmic mRNA in C. elegans embryos without affecting mRNA stability.

Multiplex DNA fluorescence in situ hybridization to analyze maternal vs. paternal C. elegans chromosomes

The paper uses multiplex DNA fluorescence in situ hybridization to study maternal and paternal chromosome organization in C. elegans, finding that paternal chromosomes adopt maternal folding patterns in embryos.

Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

The paper identifies translation-dependent mRNA localization to adherens junctions in C. elegans embryos using smFISH and mutational analyses of dlg-1 mRNA.

Neuronal control of maternal provisioning in response to social cues

This study identifies a new intergenerational communication pathway in C. elegans linking sensory perception to maternal provisioning through FMRF-like peptides.

MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

The paper shows MET-2, a methyltransferase, coordinates embryo development by distinct H3K9 methylation states, linking heterochromatin formation to loss of developmental potential.

A heterochromatic histone methyltransferase lowers nucleosome occupancy at euchromatic promoters

This study shows that H3K9me3 prevents nucleosome deposition at euchromatic promoters, affecting gene expression in C. elegans.

Regulated nuclear accumulation of a histone methyltransferase times the onset of heterochromatin formation in C. elegans embryos

The paper shows that MET-2/SETDB1 and its interactors LIN-65/ATF7IP and ARLE-14/ARL14EP regulate the timing of heterochromatin formation during C. elegans embryogenesis.