Emerging infectious diseasesGenomicsPeer-reviewed journal article
Rapid, sensitive, full genome sequencing of Severe Acute Respiratory Syndrome Virus Coronavirus 2 (SARS-CoV-2)
Clinton R. PadeniD, Ying TaoiD, Krista J. QueeniD, Jing ZhangiD, Yan LiiD, Anna UeharaiD, Suxiang TongiD
Abstract
SARS-CoV-2 recently emerged, resulting a global pandemic. Rapid genomic information is critical to understanding transmission and pathogenesis. Here, we describe validated protocols for generating high-quality full-length genomes from primary samples. The first employs multiplex RT-PCR followed by MinION or MiSeq sequencing. The second uses singleplex, nested RT-PCR and Sanger sequencing.
- organism: virus
- assay: whole-genome sequencing
- research focus: variant calling
- approach: experimental assay
- Citations
- 135
- FWCI
- 4.00
- Field percentile
- top 4%
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