Potential anti-adipogenic activity of Calligonum comosum cuminaldehyde on mouse 3T3-preadipocytes

Abstract

Obesity is a medical condition associated with serious medical and psycho-social consequences and an augmented body fat mass. Several compounds were suggested to counteract obesity and fat accumulation with variable degrees of success. Searching for a safe and effective anti-adipogenic substance, we found that cuminaldehyde-rich essential oil extracted from Calligonum comosum potentially mediate activities. The results showed that C. comosum essential oil and its major component cuminaldehyde, selectively caused significant reduction in the viability of 3T3-L1 cells when compared to fibroblasts. Furthermore, cuminaldehyde caused significant reduction in the lipid content, glucose uptake and levels of both triglycerides and cholesterol in adipocytes. Moreover, the formation of 3D-adipocyte pellets in the presence of cuminaldeyde was affected. Adipocytes matured in the presence of cuminaldehyde have significant reduction in the expression of adipocyte-specific transcripts, CAAT-enhancer binding protein alpha (CEBPa) and Peroxisome proliferator-activated receptor gamma (PPARg). Taken together, these results demonstrate a potential inhibitory role of cuminaldehyde extracted from C. comosum oil on lipid accumulation. Consequently, cuminaldeyde can be considered as a new potential anti-adipogenic agent for prevention and treatment of obesity.