bioRxivBiochemistryPreprint
Quantum dot probes for the quantitative study of drug transport by the MacAB TolC efflux pump in lipid scaffolds
H. Souabni, W. Batista dos Santos, Q. Cece, D. Puvanendran, M. Picard
Abstract
ABC tripartite efflux pumps are macromolecular membrane protein machineries that expel a large variety of drugs and export virulence factors from Gram negative bacteria. Using a lipid scaffold mimicking the two-membrane environment of the transporter and designing spectroscopic conditions allowing the monitoring of both ATP hydrolysis and substrate transport in real time, we show that MacAB-TolC accommodates transport and energy consumption with high coupling efficiency.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- The assembly and efflux processes revealed by in situ structures of the ABC-type tripartite pump MacAB-TolC
- Lipid-mediated mechanism of drug extrusion by a heterodimeric ABC exporter
Qingyu Tang, Matt Sinclair, … Hassane S. Mchaourab·bioRxiv·Feb 26, 2025
- Structural mechanisms of pump assembly and drug transport in the AcrAB-TolC efflux system
Xiaofei Ge, Zhiwei Gu, Jiawei Wang·eLifePeer-reviewed·Apr 20, 2026
- Specificity and tunability of efflux pumps: a new role for the proton gradient?
Matthew Gerry, Duncan Kirby, … Anton Zilman·PLoS Computational BiologyPeer-reviewed·Jan 27, 2025
- A lipoprotein partner for the Escherichia coli outer membrane protein TolC
Jim E. Horne, Elise Kaplan, … Ben F. Luisi·eLifePeer-reviewed·Apr 15, 2026
- Disassembly of the Escherichia coli AcrABZ-TolC efflux pump by ligand-mediated disruption of TolC-AcrA interfacial contacts
Tania Szal, Emmanouela Petsolari, … Björn Windshügel·bioRxiv·Feb 18, 2025