Engineering bacterial blight-resistant plants through CRISPR/Cas9-targeted editing of the MeSWEET10a promoter in cassava
Y. Wang, M. Geng, R. Pan, T. Zhang, X. Lu, X. Zhen, Y. Che, R. Li, J. Liu, Y. Chen, J. Guo, Y. Yao
Abstract
Cassava starch is a widely used raw materials for industrial production. However, cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) results in severe yield losses and is the most destructive bacterial disease in all worldwide cassava planting regions. This study showed that editing of the promoter in the disease-susceptibility gene MeSWEET10a of SC8 cassava confers resistance to CBB. All mutated cassava lines had normal morphological and yield-related traits as the wild type. The results lay a research foundation for breeding cassava resistant to bacterial blight. HighlightsMeSWEET10a gene in SC8 cultivar is hijacked by TALE20 from Xam11 strain. Editing of the MeSWEET10a promoter in SC8 cultivar confers resistance to CBB. All the mutated cassava lines had similar yield-related traits compared to wild-type.
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