Study on the influence of G82S RAGE polymorphism on RAGE-Amyloid interaction in AD pathology

Abstract

Receptor for advanced glycation end products (RAGE) has been implicated in the pathophysiology of AD due to its ability to bind amyloid-beta and mediate inflammatory response. G82S RAGE polymorphism is associated with AD but the molecular mechanism for this association is not understood. Our previous in silico study indicated a higher binding affinity for mutated G82S RAGE, which could be caused due to changes in N linked glycosylation at residue N81. To confirm this hypothesis, in the present study molecular dynamics (MD) simulations were used to simulate the wild type (WT) and G82S glycosylated structures of RAGE to identify the global structural changes and to find the binding efficiency with A{beta}42 peptide. Binding pocket analysis of the MD trajectory showed that cavity/binding pocket in mutant G82S glycosylated RAGE variants is more exposed and accessible to external ligands compared to WT RAGE, which can enhance the affinity of RAGE for A{beta}. To validate the above concept, an in vitro binding study was carried using SHSY5Y cell line expressing recombinant WT and mutated RAGE variant individually to which HiLyte Fluor labeled A{beta}42 was incubated at different concentrations. Saturated binding kinetics method was adopted to determine the Kd values for A{beta}42 binding to RAGE. The Kd value for A{beta}42-WT and A{beta}42-mutant RAGE binding were 92{+/-}40 nM (95% CI-52 to 152nM; R2-0.92) and 45{+/-}20 nM (95% CI -29 to 64nM; R2-0.93), respectively. The Kd value of <100nM observed for both variants implicates RAGE as a high-affinity receptor for A{beta}42 and mutant RAGE has higher affinity compared to WT. The alteration in binding affinity is responsible for activation of the inflammatory pathway as implicated by enhanced expression of TNF and IL6 in mutant RAGE expressing cell line which gives a mechanistic view for the G82S RAGE association with AD.