Loss of neuronatin increases susceptibility of SERCA to thermal inactivation

O_LIPhospholamban and sarcolipin are two key regulators of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) that have been shown to protect SERCA from thermal inactivation in muscle C_LIO_LIWe have recently detected neuronatin (NNAT) in murine skeletal muscle and have shown that it too regulates SERCA C_LIO_LIHere, we questioned whether NNAT would also protect SERCA from thermal inactivation C_LIO_LIIn response to 60 min of heat stress at 40{degrees}C, maximal SERCA activity was significantly reduced in soleus homogenates obtained from NNAT knockout mice (-23%) but not wild-type mice (-5%). C_LIO_LIOur results add further support for NNATs role in regulating SERCA function in murine muscle C_LI