Heat shock proteins research

Efficient translation of chaperones by eIF5A safeguards proteostasis under heat stress

This study shows that eIF5A facilitates efficient translation of high codon-optimal chaperones under heat stress, enhancing proteostasis and heat resistance in yeast.

Regulatory architecture tunes gene expression noise in the plant heat shock response

This study shows that the plant heat shock regulatory network controls both the magnitude and distribution of stress-responsive gene expression across cells.

Mechanism of the Sequential Trajectory of Allosteric Ring Closure in Human Chaperonin TRiC

This study uses cryo-EM and deep learning to reveal the sequential structural changes in human TRiC during ATP-induced ring closure, showing asymmetric subunit responses and coordinated domain movements.

Phase separation reshapes the conformational energy landscape of a chaperone to prime its activity

This study shows how phase separation alters the conformational dynamics of DNAJB1, priming it for HSP70 binding and stress granule disassembly.

Extracellular HSPB1 protects intestinal epithelial cells against ethanol-activated ferroptosis

This study shows extracellular HSPB1 protects intestinal epithelial cells from ethanol-induced ferroptosis but can also contribute to inflammation in chronic alcohol conditions.

Structural heterogeneity of uncrosslinked FtsH-HflKC assemblies includes variable FtsH occupancy and a distinct wild-type FtsH architecture

This study analyzes the structural heterogeneity of uncrosslinked FtsH-HflKC complexes using cryo-EM and SAXS, revealing variable FtsH occupancy and a distinct wild-type FtsH architecture.

HSF1 inhibition potentiates the antifungal activity and proteome instability induced by the fungal Hsp110 inhibitor 2H

This study shows that combining HSF1 inhibition with Hsp110 inhibitor 2H enhances antifungal activity by increasing proteome instability in fungal pathogens.

The Central Coupler of ClpXP Controls Intersubunit Communication and Couples Intrasubunit Chemical Energy with Force Generation

Q208A mutations in up to four subunits of the ClpX central coupler barely slow unfolded-substrate translocation but raise ATP spent per step, weaken force generation and can abolish GFP unfolding.

Inbred and outcrossed Drosophila embryos diverge in stress defense and developmental maintenance during heat stress

This study compares stress responses in inbred and outcrossed Drosophila embryos under heat stress, finding that inbred embryos show greater stress response activation and reduced developmental survival.

Coiled-coil homo-oligomerization and disaggregase Hsp104 act in parallel to stabilize orphan septins

The paper shows that orphan septins form coiled-coil homomers and are stabilized by Hsp104 to prevent degradation and maintain subunit stoichiometry.

e^-2 Threshold for Bistability in Non-equilibrium Sorting of Biomolecular Condensates

The paper derives a non-equilibrium model for biomolecular condensate sorting, identifying a critical Damkohler number threshold for bistability and quality control.

The cysteine-rich domain of SEP15, a selenoprotein co-chaperone of the ER chaperone, UDP-glucose:glycoprotein glucosyltransferase, adopts a novel fold

The paper determines the structure of the cysteine-rich domain of SEP15 using AlphaFold and NMR, revealing a novel helical fold stabilized by disulfide bonds.

The mitochondrial chaperone HSPD1 folds MTHFD2 independently of its co-chaperone HSPE1

This study identifies HSPD1 as a chaperone for MTHFD2, independent of HSPE1, and shows distinct functional roles for HSPD1 and HSPE1 in mitochondrial biology.

Distinct modes of folding assistance in cells by protein- and RNA-based chaperones

This study introduces CHAP-SEQ to analyze how protein- and RNA-based chaperones assist in protein folding, revealing distinct mechanisms for each type.

Chaperone isoform and interactome mapping reveals functional diversification of DNAJA2-DNAJA4 complexes via stress-regulated isoforms

The paper maps isoforms and interactions of the HSP70 network, identifying stress-regulated isoforms that form hetero-complexes and modulate TDP-43 aggregation.

Drosophila Heat Shock Factor (HSF) Regulates Developmental Growth by Maintaining the Basal Expression of HSP83/HSP90

This study shows that HSF maintains basal HSP83/HSP90 expression to support developmental growth in Drosophila, with loss of HSF causing developmental defects and reduced chaperone levels.

Small-molecule activators of the Staphylococcus aureus ClpC/ClpP AAA+ protease

The paper identifies small-molecule activators of the Staphylococcus aureus ClpC/ClpP protease through high-throughput screening and characterizes their binding sites.

Dynamic allocation of DnaK couples proteostasis demand to chaperone availability

The paper shows that DnaK, a bacterial chaperone, dynamically reallocates in response to proteostasis demand, with acute stress increasing client engagement frequency and lifetime.

Function within Disorder: Small heat shock proteins use different functional regions to chaperone tau aggregation

This study shows HSPB1 and HSPB5 use distinct N-terminal regions to delay tau aggregation, with HSPB1 under normal conditions and HSPB5 under stress.

Protective effects of heat shock protein 70 induction against global warming by using oriental bezoar and ginseng

This study shows that BG, a natural drug containing oriental bezoar and ginseng, protects against heat stress in Drosophila and human cells by upregulating HSP70 and HSF1.

Coordinated regulation of the metaboproteome by Hsp90 chaperones controls metabolic plasticity

This study shows that Hsp90 chaperones regulate the metaboproteome to control metabolic plasticity by affecting mitochondrial function and metabolic flexibility.

The chloroplast CLPD chaperone: consequences of under- and overexpression, interaction with the CLP protease core, and candidate substrates

This study investigates the role of CLPD in chloroplast proteostasis by analyzing its expression, developmental effects, and interactions with the CLP protease core and candidate substrates in Arabidopsis.

Functional diversification of two Lon homologs enhances stress adaptation in Pseudomonas aeruginosa

This study identifies AsrA, a Lon homolog in Pseudomonas aeruginosa, showing it has distinct substrate preferences and functions in stress adaptation.

Regulation of a Classical Allosteric Molecular Machine by an Intrinsically Disordered Domain: the C-termini of GroEL

This study investigates how the intrinsically disordered C-terminal tails of GroEL regulate its allosteric behavior through conformational constraints and interactions with GroES.

TRiC/CCT Chaperonin Governs RNA Polymerase II Activity in the Nucleus to Support RNA Homeostasis

This study shows TRiC/CCT regulates RNA polymerase II activity in the nucleus, affecting RNA homeostasis by modulating nascent RNA production.

Chemoproteomic profiling of Plasmodium falciparum Hsp90 inhibition reveals functional link to DNA replication pathways

The paper identifies DNA replication pathways linked to PfHsp90 inhibition in Plasmodium falciparum using chemoproteomic profiling and functional assays.

Structural and mechanistic analyses reveal collaborative regulation of HSF1 by the Hsp70-Hsp90 chaperone systems

This study reveals how HSF1 is regulated by Hsp70 and Hsp90 chaperones through structural and mechanistic analyses, showing Hsp90's role in maintaining HSF1 in a pre-activated state.

Soluble guanylyl cyclase subunits act as Hsp90 co-chaperones to ensure the expression and functional maturation of hemeproteins in mammalian cells

This study shows that sGC subunits act as Hsp90 co-chaperones to facilitate heme acquisition and functional maturation of apo-hemeproteins in mammalian cells.

Nuclear stress bodies enable a germline-specific transcriptional stress response in Drosophila

This study identifies nuclear stress bodies in Drosophila germline that mediate a stress response by delaying Hsf-dependent transcription and enabling selective expression of molecular chaperones over transposable elements.

Navigating the oligomeric landscape of the periplasmic stress response protease-chaperone DegP with charge detection mass spectrometry

This study uses charge detection mass spectrometry to analyze DegP oligomerization states and client interactions, revealing new oligomer types and dynamic behavior.