Metabolic reshaping drives novel melanin production from tryptophan in a cystic fibrosis Pseudomonas aeruginosa clinical isolate

Abstract

Pseudomonas aeruginosa is a human opportunistic pathogen, capable of producing a wide range of metabolites, including pyomelanin. This pigment results from alterations in tyrosine catabolism. Melanin synthesis from tryptophan has never been reported in Pseudomonas. In this study, we describe a tryptophan-derived melanin in P. aeruginosa PAH, a strain that was isolated from a fibrocystic patient. PAH produced a brown pigment when grown in LB or L-tryptophan-supplemented media. Structural analysis revealed this pigment was composed by two fractions differing in NaOH solubility: a soluble one consistent of pyomelanin, and an insoluble fraction with a complex structure containing substituted indolic units. A pyomelanin inhibitor enhanced total melanin synthesis, mainly the insoluble fraction, and a tryptophan 2,3-dioxygenase inhibitor decreased pigment formation. Metabolomic profiling identified distinct indolic compounds and low levels of anthranilate in PAH cultures. Genomic and transcriptomic analyses revealed the presence of mutations and downregulation of genes related to pyoverdine biosynthesis. Furthermore, iron supplementation in the culture medium reduced melanin production. Overall, tryptophan arises as a key compound for melanin production in PAH, expanding the diversity of melanins synthesized by this genus. Furthermore, iron deprivation emerges as a critical factor triggering melanin biosynthesis, probably as a survival strategy enabling persistence in the fibrocystic lung environment.