Automated sequential chromogenic IHC double staining with two HRP substrates.
Abstract
Automated IHC double staining using DAB and HRP Magenta is illustrated utilizing a new acid block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
02 /Specification & Classification
Click any tag to browse matching papers- Assay
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Multiplexed in situ protein imaging using DNA-barcoded antibodies withextended hybridization chain reactions
Y. Wang, Y. Zeng, … G. M. Church·bioRxiv·May 10, 2020
- Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections
M. M. Bolognesi, M. Manzoni, … G. Cattoretti·bioRxiv·Jun 11, 2017
- Practical quantification of immunohistochemistry antigen concentrations and reaction-diffusion parameters
Woody Perng, Bérénice Mbiribindi, … Franklin V. Peale Jr.·bioRxiv·Apr 21, 2026
- Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ hybridization
Maayan Schwarzkopf, Mike C. Liu, … Niles A. Pierce·DevelopmentPeer-reviewed·Nov 15, 2021
- Preferential cross-linking of the stereospecific complex over the encounter complexes by DOPA2, a faster cross-linker than DSS
J.-H. Wang, Z. Gong, … M.-Q. Dong·bioRxiv·Jun 6, 2022
- Serial Immunohistochemistry for High-Dimensional Single-Cell Spatial Analysis of Human Kidney Biopsies
X. J. Yang, Matthew Caleb Marlin, … Avi Z. Rosenberg·bioRxiv·Aug 12, 2026