M.-Q. Dong

ULK1-dependent phosphorylation of PKM2 antagonizes O-GlcNAcylation and inhibits the Warburg effect in breast cancer

This study identifies ULK1-mediated phosphorylation of PKM2 that inhibits O-GlcNAcylation and reduces the Warburg effect in breast cancer.

Ubiquitination-mediated Golgi-to-endosome sorting determines the toxin-antidote duality of wtf meiotic drivers

The paper investigates how ubiquitination mediates the antagonistic duality of toxin and antidote proteins in fission yeast meiotic drivers, showing that ubiquitination promotes antidote localization to endosomes and neutralizes toxin activity.

The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast

This study identifies Yep1 in fission yeast as essential for autophagosomal enclosure of ER-phagy and nucleophagy cargos, revealing its role in membrane shaping and cargo separation.

Comparative analysis of chemical cross-linking mass spectrometry data indicates that protein STY residues rarely react with N-hydroxysuccinimide ester cross-linkers

The paper evaluates the reliability of STY cross-link identifications in CXMS and finds they have high false discovery rates, often misclassified as K-K or K-mono-links.

O-GlcNAcylation promotes YTHDF1 cytosolic localization and colorectal cancer tumorigenesis

This study shows O-GlcNAcylation of YTHDF1 enhances its cytosolic localization and promotes colorectal cancer by increasing c-Myc expression.

Somatic nuclear blebbing may indicate proliferating germ cells but does not indicate organismal aging in Caenorhabditis elegans

This study examines nuclear blebbing in C. elegans, finding it accumulates with age and contributes to chromatin loss, but is not linked to aging rate or stress, and is promoted by germ cell proliferation.

PLK1 O-GlcNAcylation is essential for dividing mammalian cells and inhibits uterine carcinoma

This study identifies PLK1 O-GlcNAcylation as critical for cell division and shows its role in suppressing uterine carcinoma through biochemical and mouse model experiments.

Old Age Drives Fusion and Expansion of Vitellogenin Vesicles in the Intestine of Caenorhabditis elegans

This study examines age-related changes in vitellogenin vesicles and yolk organelles in C. elegans, finding significant expansion and fusion of these structures in post-reproductive worms.

D/E-rich peptides are less suitable than D/E-deficient peptides for identification by negative-ion HCD due to scarce production of sequencing ions from multiply charged precursors

The paper shows D/E-rich peptides produce fewer sequencing ions in negative-ion HCD MS, making them harder to identify, and suggests using proteases that generate D/E-deficient peptides for better results.

Immuno-electron microscopy localizes Caenorhabditis elegans vitellogenins along the classic exocytosis route

This study uses immuno-electron microscopy to map the subcellular localization of vitellogenins in C. elegans, showing they follow the classic exocytic pathway.

Preferential cross-linking of the stereospecific complex over the encounter complexes by DOPA2, a faster cross-linker than DSS

This study compares DOPA2 and DSS cross-linkers, finding that faster cross-linking favors the stereospecific complex over encounter complexes in transient protein interactions.

The endoplasmic reticulum membrane protein complex (EMC) negatively regulates intestinal homeostasis through the Hippo signaling pathway

This study identifies the EMC complex's role in regulating intestinal stem cell proliferation via the Hippo pathway in Drosophila, showing its negative regulation and evolutionary conservation.

Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32

The paper presents cryo-EM structures of yeast TRAPPII complex in apo and Ypt32-bound states, revealing its assembly mechanism and specific activation of Ypt31/Ypt32.

Degrading intestinal DAF-2 nearly doubles Caenorhabditis elegans lifespan without affecting development or reproduction

This study shows that reducing DAF-2 in the intestine of C. elegans nearly doubles lifespan without affecting development or reproduction.

Molecular and structural mechanisms of ZZ domain-mediated cargo recognition by autophagy receptor Nbr1

The paper identifies two new NVT cargos bound by Nbr1-ZZ1 and reveals the structural basis of ZZ domain-mediated cargo recognition in selective autophagy.

A Fast Lysine Cross-linker DOPA Enables Mass Spectrometry Analyses of Protein Unfolding and Weak Protein-protein Interactions

This study introduces a fast lysine cross-linker DOPA for mass spectrometry analyses, showing it enables rapid protein cross-linking and captures dynamic conformational changes and weak protein-protein interactions.

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger Cyclin K degradation

This paper discovers a molecular glue, HQ461, that promotes CDK12-DDB1 interaction, leading to Cyclin K degradation and cell death.

Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half assembled intermediate

The paper reports the cryo-EM structure of an early pre-60S ribosome intermediate, revealing a half-assembled subunit and key assembly factors involved in rRNA domain joining.