bioRxivMolecular BiologyPreprint
Cellular senescence and quiescence are associated with altered ribosomal RNA methylation and processing
Guohuan Yang, Clemens Heissenberger, Isabelle C. Kos-Braun, Norbert PolacekiD, Johannes GrillariiD, Markus SchossereriD, Martin KošiD
Abstract
The 2-O-methylation (2-O-Me) of ribosomal RNA (rRNA) shows plasticity potentially associated with specific cell phenotypes. We used RiboMeth-seq profiling to reveal 2-O-Me patterns specific to stress induced premature senescent (SIPS), quiescent and proliferating human dermal fibroblasts. The altered methylation levels in SIPS and quiescence partially correlated with the expression of specific snoRNAs but not fibrillarin. Senescence and quiescence were accompanied by a trend towards preferred usage of one of the two alternative ribosome biogenesis pathways.
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