bioRxivGenomicsPreprint
Residual DNA in enzymes may pose a challenge for DIY RNA sequencing protocols: how to deal
Andrey KrivoyiD, Yegor Botsmanov, Valery V. CheraneviD, Anna O. ShmitkoiD, Vera BelovaiD, Viktoriya Moskalenko, Robert AfasizheviD, Anastasia Shut, Dmitriy O. KorostiniD, Denis V. RebrikoviD
Abstract
High-throughput RNA sequencing (RNA-seq) is widely employed for gene expression profiling. This report on do-it-yourself RNA-Seq library preparation pitfalls, highlighting their correct identification and successful overcoming, may be helpful to research groups commencing a DIY RNA-Seq workflow and facing similar problems.
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