Gregor Diensthuber

FIRST-seq: a nanopore-based cDNA sequencing platform for RNA modification and structure profiling

FIRST-seq uses nanopore cDNA sequencing to profile RNA modifications and structures by analyzing RT errors, achieving single-nucleotide resolution without PCR or second-strand synthesis.

Systematic benchmarking of basecalling models for RNA modification detection with highly multiplexed nanopore sequencing

This study benchmarks RNA modification detection models using synthetic and biological data, finding high reliability but issues with false positives and cross-reactivity.

SeqTagger, a rapid and accurate tool to demultiplex direct RNA nanopore sequencing datasets

SeqTagger demultiplexes direct RNA nanopore sequencing datasets with high precision and recall, supporting multiple chemistries and library types.

Enhanced detection of RNA modifications and mappability with high-accuracy nanopore RNA basecalling models

This paper shows that using high-accuracy nanopore RNA basecalling models improves detection of RNA modifications, read mappability, and basecalling accuracy.

Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing

Nano3P-seq quantifies RNA abundance, tail composition, and tail length dynamics at single-read resolution without PCR or adapters.