Multi-Template Matching: a versatile tool for object-localization in microscopy images

Abstract

We implemented multiple template matching as both a Fiji plugin and a KNIME workflow, providing an easy-to-use method for the automatic localization of objects of interest in images. We demonstrate its application for the localization of entire or partial biological objects. The Fiji plugin can be installed by activating the Multi-Template-Matching and IJ-OpenCV update sites. The KNIME workflow can be downloaded from nodepit space or the associated GitHub repository. Python source codes and documentations are available on the following GitHub repositories: LauLauThom/MultiTemplateMatching and LauLauThom/MultipleTemplateMatching-KNIME.