Biomimetic Aorta-Gonad-Mesonephros on-a-Chip to Study Human Developmental Hematopoiesis
R. Sugimura, R. Ohta, C. Mori, A. Li, T. Mano, E. Sano, K. Kosugi, T. Nakahata, A. Niwa, M. K. Saito, Y.-s. Torisawa
Abstract
We established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from human pluripotent stem cells. We show that the AGM-chip efficiently derives endothelial-to-hematopoietic transition (EHT) in the presence of mesenchymal stroma and endothelial cells. The AGM-chip could dissect the cellular and molecular mechanisms of human developmental hematopoiesis.
02 /Specification & Classification
Click any tag to browse matching papers- Organism
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A Highly Efficient Aorta-Gonad-Mesonephros-Like Definitive Hemogenic Endothelium From Human Pluripotent Stem Cells
Yesai S. Fstkchyan, Qingwen Cheng, … Stuart M. Chambers·bioRxiv·Jun 26, 2023
- Development of a human heart-on-a-chip model using induced pluripotent stem cells, fibroblasts and endothelial cells
Yun Liu, Rumaisa Kamran, … Ken Takahashi·Scientific ReportsPeer-reviewed·Aug 8, 2024
- A Multi-Niche Microvascularized Human Bone-Marrow-on-a-Chip
M. R. Nelson, D. Ghoshal, … K. Roy·bioRxiv·Dec 15, 2019
- Analysis of Endothelial-to-Haematopoietic Transition at the Single Cell Level identifies Cell Cycle Regulation as a Driver of Differentiation
Giovanni Canu, Emmanouil I. Athanasiadis, … Ana Cvejic·Genome biologyPeer-reviewed·Jul 1, 2020
- Modeling vascular dynamics at the initial stage of endochondral ossification on a microfluidic chip using a human embryonic-stem-cell-derived organoid
Abhiraj Kesharwani, Shoichiro Tani, … Hironori Hojo·Regenerative TherapyPeer-reviewed·Dec 3, 2024
- Mesoderm-Derived PDGFRA+ Cells Regulate the Emergence of Hematopoietic Stem Cells in the Dorsal Aorta
Vashe Chandrakanthan, Prunella Rorimpandey, … John E. Pimanda·Nature Cell BiologyPeer-reviewed·Jul 28, 2022