bioRxivCell BiologyPreprint
A method for inducing a senescence trend in mesenchymal stem cells using D-galactose
Xiaoming JiiD, Xiaoyu Zhou, Tingting Chen, Chunchun Duan, Wenjing TianiD, Liyang Zhu, Yan MaiD, Liyang GaoiD
Abstract
The aging of the body is accompanied by a decline in tissue function, and this decline mainly stems from the aging of tissue stem cells. Therefore, they play a crucial role in the aging of the body.This study explores the biological characteristics of senescent hUCMSCs by establishing a replicative senescence model of hUCMSCs and a rapid senescence model induced by D - galactose (D-Gal), and analyzing the proliferative capacity, cell viability, cell cycle, pluripotency, and reactive oxygen species (ROS) levels of senescent hUCMSCs.
- organism: human
- research focus: cell type annotation
- research focus: cell signaling & trafficking
- research focus: development & morphogenesis
- research focus: metabolism & bioenergetics
- approach: experimental assay
- Citations
- 0
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Mechanical Rejuvenation of Mesenchymal Stem Cells from Aged Patients
Miles W. Massidda, Andrei Demkov, … Aaron B. Baker·bioRxiv·Jun 6, 2024
- Deconstructing replicative senescence heterogeneity of human mesenchymal stem cells at single cell resolution reveals therapeutically targetable senescent cell sub-populations
Atefeh Taherian Fard, Hannah C. Leeson, … Jessica C. Mar·bioRxiv·Jan 24, 2022
- Mitochondrial dysfunction and impaired osteogenic capacity define stress-induced osteoblast senescence
Tanja Frey, Hannah Vogg, … Jana Riegger·bioRxiv·Sep 17, 2026
- Galactose-modified duocarmycin prodrugs as senolytics
A. Guerrero, R. Guiho, … J. Gil·bioRxiv·Aug 24, 2019
- TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
C. Voskamp, L. Anderson, … R. Narcisi·European Cells and MaterialsPeer-reviewed·Nov 26, 2021
- Chemogenetic Hydrogen Sulfide Rescues Vascular Senescence in Ageing
Khatereh Shabanian, Taraneh Shabanian, … Seyed Soheil Saeedi Saravi·bioRxiv·Sep 30, 2026