Antimicrobial Agents and ChemotherapyMicrobiologyPeer-reviewed journal article
Genomic Insights into Ceftazidime Resistance in Burkholderia pseudomallei: Discovery of A172T Mutation, and Palindromic GC-Rich Repeat Sequences Facilitating penA Duplication and Amplification.
Apichai TuanyokiD, Chie NakajimaiD, Tiernan Noll, Md. Siddiqur Rahman KhaniD, Pacharapong KhrongseeiD, Charles A. YowelliD, Yu-Ping Xiao, Vanaporn WuthiekanuniD, Narisara ChantratitaiD, Henry S. HeineiD, Kuttichantran SubramaniamiD, Yasuhiko SuzukiiD, Direk LimmathurotsakuliD, Ayalew MergiaiD
Abstract
Ceftazidime (CAZ) resistance in Burkholderia pseudomallei, the causative agent of melioidosis, complicates treatment in endemic regions. This study identified a novel A172T mutation and other known penA mutations as critical contributors to CAZ resistance in a large Thai strain collection. Frequent gene duplication and amplification (GDA) of penA, likely driven by Palindromic GC-Rich Repeat Sequences (PGCRRS), highlights the urgent need for rapid diagnostics and optimized treatment strategies to manage this life-threatening disease effectively.
- organism: human
- assay: whole-genome sequencing
- research focus: drug response / drug discovery
- research focus: gene regulatory network inference
- research focus: variant calling
- approach: experimental assay
- Citations
- 2
- FWCI
- 1.38
- Field percentile
- top 19%
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