Elucidating the molecular determinants of Aβ aggregation with deep mutational scanning

Abstract

Despite the importance of A{beta} aggregation in Alzheimers disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of A{beta}40 proposed core regions important for aggregation. However, we lack even this limited mutagenesis data for the more disease-relevant A{beta}42. Thus, to better understand the molecular determinants of A{beta}42 aggregation in a cell-based system, we combined a yeast DHFR aggregation assay with deep mutational scanning. We measured the effect of 791 of the 798 possible single amino acid substitutions on the aggregation propensity of A{beta}42. We found that ~75% of substitutions, largely to hydrophobic residues, maintained or increased aggregation. We identified 11 positions at which substitutions, particularly to hydrophilic and charged amino acids, disrupted A{beta} aggregation. These critical positions were similar but not identical to critical positions identified in previous A{beta} mutagenesis studies. Finally, we analyzed our large-scale mutagenesis data in the context of different A{beta} aggregate structural models, finding that the mutagenesis data agreed best with models derived from fibrils seeded using brain-derived A{beta} aggregates.