This study investigates how N-terminal chirality and sequence variations affect the conformational landscape of A{beta}42, finding that certain mutations and chiral modulations influence the central hydrophobic core and may have protective or pathogenic effects.

Journal of Chemical Information and Modeling · Jul 30, 2026Peer-reviewedBiochemistry

Structural basis of the nucleotide incorporation cycle of bacterial DNA polymerase III

Zhi‐Qiang Xu, Slobodan Jergic, Qiang Zhu, Haibo B. Yu, Jacob S. Lewis, Simon H. J. Brown, James C. Bouwer, Harshad B. Ghodke, Peter John Lewis, Gökhan Tolun, Aaron J. Oakley, Nicholas E. Dixon

This study determines cryo-EM structures of bacterial DNA polymerase III complexes to elucidate the mechanism of nucleotide incorporation and proofreading.

bioRxiv · Jun 29, 2026 · PreprintBiochemistry

DNA barcoding for parallelised single-molecule characterisation of kinetic phenotypes

Stefan H. Mueller, David M. Grimson, Holden Paz, Aidan C. Fitch, Haibo B. Yu, Marco Ribezzi‐Crivellari, Andrew D. Griffiths, Antoine M. van Oijen, Lisanne M. Spenkelink

This paper introduces a DNA barcoding method to simultaneously profile kinetic properties of protein variants at the single-molecule level, revealing kinetic diversity and structural subpopulations.

bioRxiv · Aug 28, 2025 · PreprintBiophysics

Optimised Nanobody-based Quenchbodies for Enhanced Protein Detection

Jordan H. Cater, Nehad S. El Salamouni, Ghada H. Mansour, Sebastian Hutchinson, Conall Mc Guinness, Stefan H. Mueller, Richard R. Spinks, Nirukshan Shanmugam, Adeline Pichard-Kostuch, Viktor Zahoransky, Harshad B. Ghodke, Marco Ribezzi‐Crivellari, Haibo B. Yu, Antoine M. van Oijen, Andrew D. Griffiths, Lisanne M. Spenkelink

This study optimizes nanobody-based quenchbodies for enhanced protein detection, achieving fluorescence increases of 1.5-2.4-fold and detecting IL-6 down to 2 nM.

Communications Biology · Jun 18, 2025Peer-reviewedBioengineering