Author

Jason J. Stephany

University of Washington

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Molecular Biology· 3 Genetics· 1 Genomics· 1

Signature Assays, Organisms & Methods

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Research Focus:variant effect prediction· 4 Approach:experimental assay· 3 Organism:human· 3 Assay:proteomics· 3 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Approach:deep learning· 1

Frequent Co-Authors

Douglas M. FowleriD· 5 shared Katherine A. SitkoiD· 2 shared Kenneth A. MatreyekiD· 2 shared Melissa A. ChiassoniD· 2 shared Nicholas HasleiD· 2 shared Vanessa E. GrayiD· 2 shared Allan E. RettieiD· 1 shared Anthony Cooke· 1 shared

Activity by Year

2018-2020 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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5 shared papersMolecular Biology

Douglas M. FowlerORCID

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Author profile →

5 papers

Recent·Most cited

Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

Melissa A. Chiasson, Nathan J. Rollins, Jason J. Stephany, Katherine A. Sitko, Kenneth A. Matreyek, Marta Verby, Song Sun, Frederick P. Roth, Daniel DeSloover, Debora S. Marks, Allan E. Rettie, Douglas M. Fowler

The paper measures the effects of VKOR variants on abundance and activity in human cells, revealing its topology, active site, and the impact of human variants.

eLife · Sep 1, 2020Peer-reviewedGenomics

Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose, Nicholas A. Popp, Chris D. Richardson, Jason J. Stephany, Julie Mathieu, Cindy T. Wei, Jacob E. Corn, Dustin J. Maly, Douglas M. Fowler

This paper introduces dead-RNAs (dRNAs) to suppress unwanted CRISPR/Cas9 off-target and HDR-corrected site cleavage, enabling precise genome editing.

Nature Communications · Jun 1, 2020Peer-reviewedMolecular Biology

Visual Cell Sorting: A High-throughput, Microscope-based Method to Dissect Cellular Heterogeneity

Nicholas Hasle, Anthony Cooke, Sanjay R Srivatsan, Heather Z Huang, Jason J. Stephany, Zachary Krieger, Dana L. Jackson, Weiliang Tang, Sriram Pendyala, Raymond J. Monnat, Cole Trapnell, Emily M. Hatch, Douglas M. Fowler

This paper introduces a method to physically separate live cells based on visual phenotypes and uses it to study nuclear localization sequences and paclitaxel resistance in human cancers.

bioRxiv · Dec 9, 2019 · PreprintMolecular Biology

Elucidating the molecular determinants of Aβ aggregation with deep mutational scanning

Vanessa E. Gray, Katherine A. Sitko, Floriane Z. Ngako Kameni, Miriam Williamson, Jason J. Stephany, Nicholas Hasle, Douglas M. Fowler

The paper uses deep mutational scanning to analyze Aβ42 aggregation in yeast, identifying key residues and validating structural models.

G3 Genes Genomes Genetics · Sep 26, 2019Peer-reviewedMolecular Biology

Multiplex Assessment of Protein Variant Abundance by Massively Parallel Sequencing

Kenneth A. Matreyek, Lea M. Starita, Jason J. Stephany, Beth Martin, Melissa A. Chiasson, Vanessa E. Gray, Martin Kircher, Arineh Khechaduri, Jennifer N. Dines, Ronald J. Hause, Smita Bhatia, William Edward Evans, Mary V. Relling, Wenjian Yang, Jay Shendure, Douglas M. Fowler

This paper introduces VAMP-seq, a method to assess the abundance of protein variants, and identifies pathogenic variants in PTEN and TPMT.

Nature Genetics · May 20, 2018Peer-reviewedGenetics