Proceedings of the National Academy of SciencesMolecular BiologyPeer-reviewed journal article
MSX2 safeguards syncytiotrophoblast fate of human trophoblast stem cells
Ruth HornbachneriD, Andreas LackneriD, Henrieta PapúchováiD, Sandra HaideriD, Martin KnöfleriD, Karl MechtleriD, Paulina A. LatosiD
Abstract
The majority of placental pathologies are associated with failures in trophoblast differentiation, yet the underlying transcriptional regulation is poorly understood. Here, we use human trophoblast stem cells to elucidate the function of the transcription factor MSX2 in trophoblast specification. We show that depletion of MSX2 de-represses the syncytiotrophoblast program, while forced expression of MSX2 blocks it. We demonstrate that a large proportion of the affected genes are directly bound and regulated by MSX2 and identify components of the SWI/SNF complex as its strong interactors. Our findings uncover the pivotal role of MSX2 in cell fate decisions that govern human placental development and function.
- organism: human
- assay: bulk RNA-seq
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: statistical model
- approach: experimental assay
- Citations
- 58
- FWCI
- 8.82
- Field percentile
- top 1%
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