bioRxivMolecular BiologyPreprint
CUT&ID for simultaneous profiling of protein-DNA and protein-protein interactions
Abstract
We developed CUT&ID to simultaneously profile the native genomics and proteomics associations of any endogenous target, in a single streamlined workflow with no need of transgenesis. CUT&ID is enabled by a fusion protein that drives sequential target recognition, proximal protein biotinylation and DNA cleavage in living cells. CUT&ID is broadly applicable and yields high signal-to-noise genomics and proteomics identification across all types of gene regulators tested.
- assay: protein structure
- assay: DNA methylation
- research focus: protein structure prediction
- research focus: protein design
- approach: experimental assay
- Citations
- 0
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