Nature CommunicationsGeneticsPeer-reviewed journal article
Targeting Repair Pathways With Small Molecules Increases Precise Genome Editing In Pluripotent Stem Cells
Abstract
Introductory paragraph Introductory paragraph Main text Author contributions Competing Interests Cell culture Generation and validation of... Small molecules Design of gRNAs and... Lipofection of oligonucleotides Ribonucleoprotein nucleofection FACS-sorting Illumina library preparation and... CRISPResso analysis Statistical analysis Online Methods References Online methods references A now frequently used method to edit mammalian genomes uses the nucleases CRISPR/Cas9 and CRISPR/Cpf1 or the nickase CRISPR/Cas9n to introduce double-strand breaks (DSBs) which are then repaired ...
- organism: human
- assay: whole-genome sequencing
- research focus: variant calling
- approach: experimental assay
- Citations
- 174
- FWCI
- 7.29
- Field percentile
- top 2%
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