bioRxivBioengineering
No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice
Abstract
Introduction Introduction Results and Discussion Conclusion Author Contributions Competing Financial Interests 1. gRNA choices and... 2. Zygote injection 3. DNA extraction from... 4. Amplicon sequencing and... 5. Whole Genome Sequencing 6. Probability of detecting... 7. Variant calling, Trio... 8. Intersection of all... 9. Comparison of SNVs... 10. Validation with PCR... 11. Re-analysis of De... Supplementary Figure Supplementary tables References CRISPR-Cas technologies have transformed genome-editing of experimental organisms and have ...
- organism: mouse
- assay: whole-genome sequencing
- research focus: variant calling
- approach: experimental assay
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