Kendell Clement

This study enhances CAR-NK cell functionality using non-viral base editing and transposon integration, showing improved therapeutic potential in Burkitt's lymphoma models.

This study shows that gene editing of HSCs without ex vivo culture reduces genotoxicity while maintaining therapeutic efficacy for beta-hemoglobinopathy treatment.

The paper develops ABSOLVE-seq to assess genome editing off-targets linked to genetic variants using primary cells and sequencing, enabling evaluation of human genetic diversity's impact on gene editing specificity.

ONE-seq identifies population-specific off-target effects of CRISPR gene editors using global human genome sequence diversity.

The paper identifies DNA methylation as a key mechanism for maintaining monoallelic expression on autosomes and reveals its tunable control of allele-specific expression.

The paper benchmarks 10 scATAC-seq analysis methods on synthetic and real datasets, finding SnapATAC, Cusanovich2018, and cisTopic perform best in separating cell populations across varying coverage and noise levels.

CRISPResso2 is a software tool for analyzing genome editing data, providing allele-specific quantification and scalable alignment to assess editing efficiency and safety.

CRISPR-SURF deconvolves CRISPR tiling screen data to identify functional regulatory elements across CRISPR-Cas, CRISPRi, and CRISPRa experiments.

The paper introduces VIVO, a method to detect off-target mutations from in vivo CRISPR-Cas editing, showing that well-designed gRNAs can edit efficiently without detectable off-targets.

The paper introduces AmpUMI, a tool for designing and analyzing UMI-based amplicon sequencing experiments, providing a model to determine optimal UMI length and quality control features.

The paper challenges the claim that CRISPR-Cas9 induces off-target mutations by showing that the observed variants are likely pre-existing genetic differences between treated mice and the control.

This paper evaluates CRISPR-Cas9 base editors to minimize bystander and off-target mutations while improving precision in C-to-T DNA editing.