bioRxivMicrobiology
In-vivo detection of cyclic-di-AMP in Staphylococcus aureus
N. Mukkayyan, R. Poon, P. Sander, L.-Y. Lai, Z. Zubair-Nizami, M. Hammond, S. S. Chatterjee
Abstract
Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in Staphylococcus aureus. In this study, we developed a novel biosensor (bsuO P6-4) for in-vivo detection of CDA in S. aureus. Our study showed that bsuO P6-4 could detect a wide concentration range of CDA in both laboratory and clinical strains making it suitable for use in both basic and clinical research applications.
- organism: bacteria (other)
- research focus: host-pathogen & immune response
- approach: experimental assay
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