bioRxivMolecular BiologyPreprint
RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions
Julie M. WellsiD, Anuj SrivastavaiD, Richard S. Maser, Rupesh K. KesharwaniiD, Vishal SarsaniiD, Sandra L Daigle, Ryan P. Lynch, Heidi J. Munger, Rosalinda A. Doty, Ruth L. Saxl, Carol J. BultiD
Abstract
MicroRNAs (miRNAs) are crucial post-transcriptional regulators of gene expression in species ranging from plants to mammals. Unraveling the complex networks of miRNA-mediated regulation of gene expression requires the ability to identify the mRNA targets of individual miRNAs in native cellular contexts. Here we describe RIP-SIR, an unbiased genome-wide method for identifying interactions between endogenous miRNAs and their targets in almost any tissue or cell type.
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