Sequential 3D OrbiSIMS and LESA-MS/MS-based metabolomics for prediction of brain tumor relapse from sample-limited primary tissue archives
J. Meurs, D. J. Scurr, A. Lourdusamy, L. C. D. Storer, R. G. Grundy, M. R. Alexander, R. Rahman, D.-H. Kim
Abstract
We present here a novel surface mass spectrometry strategy to perform untargeted metabolite profiling of formalin-fixed paraffin-embedded (FFPE) pediatric ependymoma archives. Sequential Orbitrap secondary ion mass spectrometry (3D OrbiSIMS) and liquid extraction surface analysis-tandem MS (LESA-MS/MS) permitted the detection of 887 metabolites (163 chemical classes) from pediatric ependymoma tumor tissue microarrays (diameter View larger version (11K): org.highwire.dtl.DTLVardef@1f9c850org.highwire.dtl.DTLVardef@1ce0568org.highwire.dtl.DTLVardef@c5023borg.highwire.dtl.DTLVardef@15ad6e_HPS_FORMAT_FIGEXP M_FIG For Table of Contents Only C_FIG
02 /Specification & Classification
Click any tag to browse matching papersReproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Ambient mass spectrometry imaging enables spatial metabolomics of optimal cutting temperature compound (OCT)-embedded tumors
Joseph Monaghan, Nicholas Woytowich, … Kyle D. Duncan·bioRxiv·Nov 5, 2025
- Deep Learning Outperforms Classical Machine Learning Methods in Pediatric Brain Tumor Classification through Mass Spectra
Thais Maria Santos Bezerra, Matheus Silva de Deus, … João Meidânis·Intelligence-Based MedicinePeer-reviewed·Jan 1, 2024
- Matrix selection for the visualization of small molecules and lipids in brain tumors using untargeted MALDI-TOF mass spectrometry imaging
Tianyao Lu, Lutz Freytag, … Saskia Freytag·MetabolitesPeer-reviewed·Nov 9, 2023
- Absolute quantification of cerebral metabolites using 2D 1H-MRSI with quantitative MRI-based water reference
Dennis C. Thomas, Şeyma Alçiçek, … Ulrich Pilatus·bioRxiv·Nov 25, 2024
- A Survey of Orbitrap All Ion Fragmentation Analysis Assessed by an R MetaboList Package to Study Small-molecule Metabolites
Enrique Sentandreu, Manuel David Peris‐Díaz, … Stefano Tiziani·ChromatographiaPeer-reviewed·May 11, 2018
- Deep Learning-based Pseudo-Mass Spectrometry Imaging Analysis for Precision Medicine
Xiaotao Shen, Wei Shao, … Michael P. Snyder·Briefings in BioinformaticsPeer-reviewed·Aug 10, 2022