Sören Doose

Programmable protein reference standards for benchmarking sub-10-nm fluorescence microscopy

This paper introduces programmable protein standards for benchmarking sub-10-nm fluorescence microscopy, demonstrating their utility in assessing fluorophore interactions and imaging performance.

Nanoscale imaging of expanded cells and proteins with spontaneously blinking dyes

This paper introduces a single-step expansion microscopy method enabling SMLM with spontaneously blinking dyes in expanded samples, achieving nanometer-resolution imaging of cellular structures.

Single-molecule localization microscopy reveals the molecular organization of endogenous membrane receptors

The paper develops an optimized immunolabeling protocol to quantify endogenous membrane receptor stoichiometry using single-molecule localization microscopy on Jurkat T cells and primary multiple myeloma cells.

Resolving endogenous protein organization in cells with nanometer precision

The paper develops an optimized Ex-dSTORM method to resolve endogenous protein assemblies with nanometer precision in cells.

RBM20-variants induce distinct calcium handling and metabolic phenotypes in patient-specific stem cell models of dilated and non-compaction cardiomyopathy

This study compares patient-specific stem cell models of RBM20 mutations causing dilated cardiomyopathy and left ventricular non-compaction, identifying distinct molecular, cellular, and functional impairments.

Coronaviruses use ACE2 monomers as entry receptors

This study shows that SARS-CoV-2 uses monomeric ACE2 receptors for infection, with single S protein interactions sufficient for entry.

Dynamic remodeling of ribosomes and endoplasmic reticulum in axon terminals of motoneurons

The paper investigates dynamic remodeling of endoplasmic reticulum and ribosomes in axons of motoneurons in response to BDNF stimulation, revealing their role in local translation and synapse maintenance.

Active zone compaction in presynaptic homeostatic potentiation

The paper investigates presynaptic homeostatic potentiation (PHP) in Drosophila melanogaster, finding that AZs compact rather than enlarge, increasing protein density without changing Brp copy numbers.