Click-ExM enables expansion microscopy for all biomolecules
D.-e. Sun, X. Fan, H. Zhang, Z. Huang, Q. Tang, W. Li, J. Bai, X. Lei, X. Chen
Abstract
Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM, which integrates click-labeling into ExM to enable a "one-stop-shop" method for nanoscale imaging of various types of biomolecules. Using 18 clickable labels for click-ExM imaging of DNA, RNA, proteins, lipids, glycans and small molecules, we demonstrate its universality, compatibility with signal-amplification techniques, and broad applications in cellular imaging.
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