Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures

Gabriela J. Blaszczyk; Valerio E. C. Piscopo; Taylor Goldsmith; Alexandra Chapleau; Julien Sirois; Geneviève Bernard; Jack Perry Antel; Thomas M. Durcan

doi:10.1038/s41540-025-00515-z

Neurosciencenpj Systems Biology and ApplicationsApr 15, 2025

Peer-reviewed journal article

Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures

Gabriela J. Blaszczyk iD, Valerio E. C. Piscopo iD, Taylor Goldsmith iD, Alexandra Chapleau iD, Julien Sirois iD, Geneviève Bernard iD, Jack Perry Antel iD, Thomas M. Durcan iD

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Abstract

Cellular heterogeneity is a common issue in differentiation protocols of oligodendrocytes (OLs) from human induced pluripotent stem cells. Our previous work described a novel method to generate OLs and highlighted the presence of glial progenitors. Here, we unravel the glial heterogeneity and characterize the response of isolated subpopulations to differentiation. This study provides a novel tool for studying the dynamics of glial development in vitro and on a transcriptomic level.