Large-scale cellular-resolution imaging of neural activity in freely behaving mice

Daniel P. Leman; Anderson Chen; Kevin A. Bolding; J. Tai; Lucius K. Wilmerding; Howard J. Gritton; Yarden Cohen; William Yen; L. Nathan Perkins; William A. Liberti; Kıvılcım Kılıç; Xue Han; Alberto Cruz‐Martín; Timothy J. Gardner; Timothy M. Otchy; Ian G. Davison

doi:10.1101/2021.01.15.426462

NeurosciencebioRxivJan 15, 2021

Preprint

Large-scale cellular-resolution imaging of neural activity in freely behaving mice

Daniel P. Leman iD, Anderson Chen iD, Kevin A. Bolding iD, J. Tai, Lucius K. Wilmerding iD, Howard J. Gritton iD, Yarden Cohen iD, William Yen iD, L. Nathan Perkins iD, William A. Liberti, Kıvılcım Kılıç iD, Xue Han iD, Alberto Cruz‐Martín iD, Timothy J. Gardner iD, Timothy M. Otchy iD, Ian G. Davison iD

Abstract

Miniaturized microscopes for head-mounted fluorescence imaging are powerful tools for visualizing neural activity during naturalistic behaviors, but the restricted field of view of first-generation miniscopes limits the size of neural populations accessible for imaging. Here we describe a novel miniaturized mesoscope offering cellular-resolution imaging over areas spanning several millimeters in freely moving mice. This system enables comprehensive visualization of activity across entire brain regions or interactions across areas.

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