bioRxivBiophysics
Alzheimer’s Amyloid-Beta Intermediates Generated by Polymer-Nanodiscs
B. Sahoo, T. Genjo, M. Bekier, S. J. Cox, A. Stoddard, M. Ivanova, K. Yasuhara, C. A. Fierke, Y. Wang, A. Ramamoorthy
Abstract
Polymethacrylate-copolymer (PMA) encased lipid-nanodiscs (~10 nm) and macro-nanodiscs (>15 nm) are used to study A{beta}1-40 aggregation. We demonstrate that PMA-nanodiscs form a ternary association with A{beta} and regulate its aggregation kinetics by trapping intermediates. Results demonstrating reduced neurotoxicity of nanodisc-bound A{beta} oligomers are also reported.
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