BiochimieMolecular BiologyPeer-reviewed journal article
Old Dog, New Tricks: Influenza A Virus NS1 and In Vitro Fibrillogenesis
Aram A. ShaldzhyaniD, Yana A. ZabrodskayaiD, Irina L. BaranovskayaiD, Maria V. SergeevaiD, Andrey Nikolaevich GorshkoviD, I.I. Savin, Sergey M. ShishlyannikoviD, Edward Smith RamsayiD, Aleksandr V. ProtasoviD, A.P. Kukhareva, Vladimir V. EgoroviD
Abstract
The influenza NS1 protein is involved in suppression of the host immune response. Recently, there is growing evidence that prion-like protein aggregation plays an important role in cellular signaling and immune responses. In this work, we obtained a recombinant, influenza A NS1 protein and showed that it is able to form amyloid-like fibrils in vitro. Using proteolysis and subsequent mass spectrometry, we showed that regions resistant to protease hydrolysis highly differ between the native NS1 form (NS1-N) and fibrillar form (NS1-F); this indicates that significant structural changes occur during fibril formation. The discovery of the ability of NS1 to form amyloid-like fibrils may be relevant to uncovering relationships between influenza A infection and modulation of the immune response.
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- top 22%
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