Raman spectral analysis of microbial pigment compositions in vegetative cells and heterocysts of multicellular cyanobacterium

Abstract

The one-dimensional multicellular cyanobacterium, Anabaena sp. PCC 7120, exhibits a simple topology consisting of two types of cells under the nitrogen-depleted conditions. Although the differentiated (heterocyst) and undifferentiated cells (vegetative cells) were distinguished by their cellular shapes, we found that their internal states, that is, microbial pigment compositions, were distinguished by using a Raman microscope. Almost of Raman bands of the cellular components were assigned to vibrations of the pigments; chlorophyll a, {beta}-carotene, phycocyanin, and allophycocyanin. We found that the Raman spectral measurement can detect the decomposition of both phycocyanin and allophycocyanin, which are components of the light-harvesting phycobilisome complex in the photosystem II. We observed that the Raman bands of phycocyanin and allophycocyanin exhibited more remarkable decrease in the heterocysts when compared to those of chlorophyll a and {beta}-carotene. This result indicated the prior decomposition of phycobilisome in the heterocysts. Moreover, the Raman bands of allophycocyanin were more decreased in heterocysts when compared to those of phycocyanin, suggesting that the decomposition of phycocyanin was more strongly suppressed than allophycocyanin in heterocysts. We show that the Raman measurement is useful to detect the change of pigment composition in the cell differentiation.