bioRxivCell BiologyPreprint
FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting
N. A. Djaja, T. Bracha, S. B. Yu, H. Wang, N. M. Carlson, G. Pekkurnaz
Abstract
Plate-based quantitative metabolic flux analysis has emerged as the central technology to examine cellular metabolism and mitochondrial bioenergetics. However, accurate interpretation of metabolic activity between different experimental conditions in multi-well microplates requires data normalization based on in situ cell counts. Here, we describe FluxNorm, a platform-independent semi-automated computational workflow, validated for three different cell types, to normalize cell density for accurate assessment of cellular bioenergetics.
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