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bioRxivCancer Biology

Comprehensive Analysis of Spatial Architecture in Primary Liver Cancer

R. Wu, W. Guo, X. Qiu, S. Wang, C. Sui, Q. Lian, J. Wu, Y. Shan, Z. Yang, S. Yang, T. Wu, K. Wang, Y. Zhu, S. Wang, C. Liu, Y. Zhang, B. Zheng, Z. Li, Y. Zhang, S. Shen, Y. Zhao, W. Wang, J. Bao, J. Hu, X. Wu, , L. Chen

PDFDOIPreprint

Abstract

Heterogeneity is the major challenge for cancer prevention and therapy. Here, we firstly constructed high-resolution spatial transcriptomes of primary liver cancers (PLCs) containing 84,823 spots within 21 tissues from 7 patients. The sequential comparison of spatial tumor microenvironment (TME) characteristics from non-tumor to leading-edge to tumor regions revealed that the tumor capsule potentially affects intratumor spatial cluster continuity, transcriptome diversity and immune cell infiltration. Locally, we found that the bidirectional ligand-receptor interactions at the 100 m wide cluster-cluster boundary contribute to maintaining intratumor architecture. Our study provides novel insights for diverse tumor ecosystem of PLCs and has potential benefits for cancer intervention.

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