Nature CommunicationsGenomicsPeer-reviewed journal article
Single-nucleus RNA-seq2 reveals a functional crosstalk between liver zonation and ploidy
Maria Lucia RichteriD, Ioannis K. DeligiannisiD, Kelvin YiniD, Anna DaneseiD, E. Lleshi, Paul G. CouplandiD, Catalina A. VallejosiD, Kylie P. MatchettiD, Neil Cowan HendersoniD, Maria Colomé‐TatchéiD, Celia Pilar Martinez‐JimeneziD
Abstract
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity we have developed a single-nucleus RNA-seq2 method that allows deep characterization of nuclei isolated from frozen archived tissues. We have used this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy, and have discovered that gene expression in tetraploid mononucleated hepatocytes is conditioned by their position within the hepatic lobe. Our work has revealed a remarkable crosstalk between gene dosage and spatial distribution of hepatocytes.
- organism: mouse
- assay: scRNA-seq
- research focus: cell type annotation
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: statistical model
- Citations
- 75
- FWCI
- 5.02
- Field percentile
- top 3%
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