henhaobio
Sign in

bioRxivMolecular BiologyPreprint

Single species RNA purification with DNA nanoswitches

Lifeng ZhouiD, Cassandra CavaliereiD, Andrew Hayden, Paromita Dey, Song Mao, Arun Richard ChandrasekaraniD, Jia ShengiD, Bijan K. DeyiD, Ken HalvorseniD

PDFDOI

Abstract

We report a novel method to purify individual RNA species using programmable DNA nanoswitches to facilitate RNA capture, release, and isolation. We validate sequence-based purification of microRNA, rRNA, and an mRNA fragment from total RNA, demonstrate multiplexing with two different RNA species, and show an application with downstream LC/MS analysis to identify RNA modifications. The simplicity, low cost, and low sample requirements of our method make it suitable for easy adoption.Competing Interest StatementLZ, ARC, and KH have intellectual property related to DNA nanoswitches. All other authors declare no competing interests.View Full Text

Citations
0

Reproducibility ledger

Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.

Sign in to file a reproducibility report.

Related papers

Similarity Map
SelectedSame fieldCross-field
THISDas '23Vries '26Tan '19Chandraseka '18Bošković '26Horikawa '26
67% semantic matchMolecular Biology
Aniruddha Das, Debojyoti Das, Arundhati Das+1 more·Wellcome Open Research·Mar 23, 2023· 4 citations