Freshwater Blackworms Solving a Maze
Abstract
We investigated the behaviour of freshwater blackworms (\emph{Lumbriculus variegatus}) in a circular maze containing food in its central chamber. Analysis of 11 time-lapse recordings showed that all tested worms entered the central chamber, although first-arrival times and spatial exploration patterns varied substantially. Registered occupancy maps revealed non-uniform use of corridors and chambers. Because the study did not include a no-food control, the observations demonstrate central-chamber entry and spatial exploration but do not by themselves establish food localisation or chemotaxis. The assay provides a reproducible framework for studying annelid exploration and for future controlled comparisons of sensory cues and maze complexity.
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