sangeranalyseR: simple and interactive analysis of Sanger sequencing data in R
Kuan-Hao ChaoiD, Kirston BartoniD, Sarah PalmeriD, Robert LanfeariD
Abstract
SummarysangeranalyseR is an interactive R/Bioconductor package and two associated Shiny applications designed for analysing Sanger sequencing from data from the ABIF file format in R. It allows users to go from loading reads to saving aligned contigs in a few lines of R code. sangeranalyseR provides a wide range of options for a number of commonly-performed actions including read trimming, detecting secondary peaks, viewing chromatograms, and detecting indels using a reference sequence. All parameters can be adjusted interactively either in R or in the associated Shiny applications. sangeranalyseR comes with extensive online documentation, and outputs detailed interactive HTML reports. Availability and implementationsangeranalyseR is implemented in R and released under an MIT license. It is available for all platforms on Bioconductor (https://bioconductor.org/packages/sangeranalyseR) and on Github (https://github.com/roblanf/sangeranalyseR). Contactkuanhao.chao@gmail.com Supplementary informationDocumentation at https://sangeranalyser.readthedocs.io/.
02 /Specification & Classification
Click any tag to browse matching papers- Citations
- 63
- FWCI
- 2.43
- Field percentile
- top 9%
- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- regionReport: Interactive reports for region-based analyses
Leonardo Collado-Torres, Andrew E Jaffe, Jeffrey T Leek·F1000ResearchPeer-reviewed·May 1, 2015
- NGseqBasic - a single-command UNIX tool for ATAC-seq, DNaseI-seq, Cut-and-Run, and ChIP-seq data mapping, high-resolution visualisation, and quality control
Jelena M. Telenius, Jim R. Hughes·bioRxiv·Aug 16, 2018
- ngsReports: An R Package for managing FastQC reports and other NGS related log files.
Christopher M. Ward, Thu‐Hien To, Stephen Pederson·BioinformaticsPeer-reviewed·Dec 12, 2019
- ascend: R package for analysis of single cell RNA-seq data
Anne Senabouth, Samuel W. Lukowski, … Joseph E. Powell·GigaSciencePeer-reviewed·Aug 1, 2019
- TraceTrack, an Open-Source Software for Batch Processing, Alignment and Visualization of Sanger Sequencing Chromatograms
Kveta Brazdilova, David Příhoda, … Danny A. Bitton·Bioinformatics AdvancesPeer-reviewed·Jan 1, 2023
- HTSeq - A Python framework to work with high-throughput sequencing data
Simon Anders, Paul Theodor Pyl, Wolfgang Huber·BioinformaticsPeer-reviewed·Sep 25, 2014