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BioinformaticsComputational and Structural Biotechnology Journal
Peer-reviewed journal article

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

Adam Mark BlanchardiD, Adam R. TayloriD, Andrew WarryiD, Freya Shephard, Alice CurweniD, James A. LeighiD, Richard David EmesiD, Sharon A. EganiD

Abstract

MotivationCurrent methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilitate real time analysis. SummaryWe have created an accessible web-based browser tool for visualisation and downstream analysis of high-throughput TIS data. This includes multiple interactive and sortable tables to aid the user to identify genes of interest, enabling the user to gain a greater understanding of the genes contributing to fitness in their experimental work. PIMMS-Dash permits researchers, with any level of bioinformatics knowledge, to interrogate data sets and generate publication quality figures. AvailabilityPIMMS-Dash is freely available and is accessible online at https://pimms-dashboard-uon.azurewebsites.net

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