Cells stably expressing shRNA against MYO10 display altered cell motility

Joanna A. Mas; Chase E. Cristella; Vu Phan; Lauren Wendt; Charlotte A. Rose; Abigail Ali; David F. Carpio; Christine Cole; Paige Embley; Jack E. Hoskins-Harris; Delia Johnson; Noelle Ledoux; Hannah W. Lwin; Sarah Salah; Erin Weisbart; Stacey J Criswell; Omar A. Quintero

doi:10.1101/2025.07.23.666455

Cell BiologybioRxivJul 23, 2025

Preprint

Cells stably expressing shRNA against MYO10 display altered cell motility

Joanna A. Mas, Chase E. Cristella iD, Vu Phan, Lauren Wendt iD, Charlotte A. Rose iD, Abigail Ali, David F. Carpio, Christine Cole, Paige Embley, Jack E. Hoskins-Harris iD, Delia Johnson, Noelle Ledoux iD, Hannah W. Lwin iD, Sarah Salah iD, Erin Weisbart iD, Stacey J Criswell iD, Omar A. Quintero iD

Abstract

Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10s cellular roles, we generated MYO10 knockdown (MYO10KD) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10KD lines showed reduced proliferation and impaired cell migration in wound assays. There were fewer edge filopodia in HeLa cells. Additionally, MYO10KD cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10s importance in cell proliferation, adhesion, and migration; these MYO10KD lines provide an accessible cell culture model for further study of MYO10.

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