Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques
Zhenyu LiiD, Matthew D. J. DicksiD, Louisa M. Rose, Rebecca A RusselliD, Katherine McMahaniD, Ninaad LasradoiD, Joseph P. NkololaiD, Erica N. Borducchi, Jinyan Liu, Jayeshbhai ChaudhariiD, Sumi BiswasiD, Dan H. BarouchiD
Abstract
Adenovirus vector-based vaccines were deployed widely during the COVID-19 pandemic. In this study, we explored the potential of displaying an antigen on the surface of the adenovirus capsid as well as encoding an antigen as a transgene in the adenovirus vector to optimize both humoral and cellular immune responses. We show that displaying SARS-CoV-2 Spike receptor biding domain (RBD) on the Ad5 capsid while simultaneously encoding Spike as a transgene induced robust antibody and T cell responses in rhesus macaques. These data demonstrate that adenoviruses can be utilized simultaneously as both nanoparticle scaffolds and viral vectors.
02 /Specification & Classification
Click any tag to browse matching papers- Citations
- 1
- FWCI
- 2.68
- Field percentile
- top 12%
- Organism
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Prime-boost vaccination of mice and Rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates
- Adenoviral-Based Vaccine Elicits Robust Systemic and Mucosal Cross-Reactive Responses in African Green Monkeys and Reduces Shedding after SARS-CoV-2 Challenge
S. N. Tedjakusuma, C. A. Lester, … B. A. Flitter·bioRxiv·Dec 19, 2022
- Complete Protection of Nasal and Lung Airways Against SARS-CoV-2 Challenge by Antibody Plus Th1 Dominant N- and S-Specific T-Cell Responses to Subcutaneous Prime and Thermally-Stable Oral Boost Bivalent hAd5 Vaccination in an NHP Study
E. Gabitzsch, J. T. Safrit, … P. Soon-Shiong·bioRxiv·Dec 8, 2020
- Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat RBD of SARS-CoV-2 spike protein protects mice against COVID-19
- Comparison of Mucosal and Intramuscular Immunization against SARS-CoV-2 with Replication-Defective and Replicating Single-cycle Adenovirus Vaccines
M. A. Barry, H. Mudrick, … G. Olivier·bioRxiv·Apr 20, 2021
- Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization using a versatile adenovirus-inspired multimerization platform
C. Chevillard, A. Amen, … P. Fender·bioRxiv·Sep 13, 2021