bioRxivGeneticsPreprint
Engineering Threshold-Based Selection Systems
Katherine H. Pedone, Vanessa González‐Pérez, Luciana E. Leopold, Channing J. DeriD, Adrienne D. CoxiD, Shawn Cameron AhmediD, David J. ReineriD
Abstract
Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt C. elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.
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