Colonization at birth with human CST IV cervicovaginal microbiota alters development and increases neonatal mortality in mice

Abstract

Profound racial health disparities contribute to maternal-infant morbidity and mortality. An emergent risk factor is the maternal microbiota, whereby compositional alterations impact maternal health and offspring development during pregnancy and beyond. The presence of a nonoptimal CST IV cervicovaginal microbiota, more common in Black and Hispanic women, is associated with increased risk of preterm birth and adverse birth outcomes. Through examination of the biological mechanisms by which vertical transmission of microbiota from mother to offspring influences postnatal development, we found that exposing cesarean delivered mice with CST IV cervicovaginal microbiota from pregnant women produced lasting effects on offspring metabolic, immune, and neural outcomes. We then examined how compounding effects of a typical high-risk, proinflammatory in utero environment, characterized by a maternal obesogenic state and the presence of G. vaginalis, would affect the offspring response to CST IV microbial gut colonization. The resultant developmental immaturity, coupled with an exaggerated immune response induced by exposure to risk-associated maternal microbiota, resulted in a profound increase in neonatal mortality, supporting the critical importance of elucidating the multifactorial biological mechanisms involved in high-risk pregnancies. HighlightsO_LIGut colonization by nonoptimal human cervicovaginal microbiota had sex-specific effects on postnatal development. C_LIO_LIA high-risk in utero environment increased offspring mortality risk. C_LIO_LIHeightened innate immune response associated with mortality risk. C_LIO_LIDevelopmental immaturity, premature birth, and exposure to CST IV contribute to increased offspring mortality risk. C_LI